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ORIGINAL ARTICLE
Year : 2018  |  Volume : 1  |  Issue : 4  |  Page : 136-141

Expression and tumor-promoting effects of caprin-1 in human glioma


1 Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China
2 Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China; Department of Surgery, University of British Columbia, Vancouver, Canada
3 Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China; Biomedical Research Centre, University of British Columbia, Vancouver, Canada

Correspondence Address:
Dr. Bin Wang
Biomedical Research Centre, The University of British Columbia, 2222 Health Sciences Mall, Vancouver, BC, V6TZ3, Canada

Dr. Jie Luo
Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, 32 South Renmin Road, Shiyan 442000, Hubei
China
Dr. Long-Jun Dai
Department of Surgery, University of British Columbia, 400-828 West 10th. Avenue, Vancouver, BC, V5ZL8, Canada

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/glioma.glioma_29_18

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Background: Cytoplasmic activation/proliferation-associated protein-1 (caprin-1) is a newly discovered RNA-binding protein and is now recognized as one of the putative oncogenes. This study was performed to reveal its presence in human gliomas and its oncogenic functions in human glioblastoma-derived Denver brain tumor research group 05 (DBTRG-05MG) cells. Materials and Methods: Clinical glioma samples were cumulatively collected for the identification of caprin-1 using immunoblot analysis and immunofluorescence detection. DBTRG cells transfected with caprin-1-specific small interfering RNA (siRNA) were used to verify caprin-1's oncogenic function using a real-time cell analyzer (RTCA) and scratch assay. Results: Seven of eight collected glioma samples were identified as positive for caprin-1 expression. siRNA dose-responsive inhibition of cell proliferation was observed in DBTRG cells with RTCA, and cell migration rate was significantly reduced by siRNA transfection (P < 0.05). Conclusion: The present study identified the higher expression of caprin-1 in human glioblastoma-derived DBTRG cells. Its oncogenic functions, mainly enhanced cell proliferation and promoted cell migration capacity, were also verified in these cells. This study provided fundamentals for developing caprin-1 as a therapeutic target for the treatment of gliomas.


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