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  Indian J Med Microbiol
 

Figure 1: Effects of SLC22A18 upregulation and SATB1 downregulation on U251 cell survival, apoptosis, and proliferation in vitro and in vivo. (A) U251 cell survival in vitro. Cell survival was assessed by the MTT assay. Cell survival rates are expressed relative to those of the control U251 cells (control value, 100%). Each assay was performed at least three times. (B) U251 cell apoptosis in vitro and in vivo. Apoptosis of the glioma cells, in vitro and in vivo, was detected and quantified using the Guava Nexin assay at 3 days. (C) U251 cell proliferation in vitro and in vivo. Ki67 was determined as a parameter of cell proliferation activity. Tumor cells were stained with Ki67 antibody (1:100) using immunocytochemistry. Data are expressed as the mean ± standard deviation, and analyzed using the Student's t-test. *P < 0.05, **P < 0.01, vs. U251 group

Figure 1: Effects of <i>SLC22A18</i> upregulation and <i>SATB1</i> downregulation on U251 cell survival, apoptosis, and proliferation <i>in vitro</i> and <i>in vivo</i>. (A) U251 cell survival <i>in vitro.</i> Cell survival was assessed by the MTT assay. Cell survival rates are expressed relative to those of the control U251 cells (control value, 100%). Each assay was performed at least three times. (B) U251 cell apoptosis <i>in vitro</i> and <i>in vivo.</i> Apoptosis of the glioma cells, <i>in vitro</i> and <i>in vivo</i>, was detected and quantified using the Guava Nexin assay at 3 days. (C) U251 cell proliferation <i>in vitro</i> and <i>in vivo</i>. Ki67 was determined as a parameter of cell proliferation activity. Tumor cells were stained with Ki67 antibody (1:100) using immunocytochemistry. Data are expressed as the mean ± standard deviation, and analyzed using the Student's <i>t</i>-test. *<i>P</i> < 0.05, **<i>P</i> < 0.01, <i>vs</i>. U251 group